Molecular cloning and expression of the human and mouse homologues of the Drosophila dachshund gene |
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Authors: | Zbynek Kozmik Peter Pfeffer Jarmila Kralova J Paces Vaclav Paces Anna Kalousova A Cvekl |
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Institution: | (1) Institute of Molecular Genetics, Flemingovo 2, CZ-16637 Praha 6, Czech Republic,;(2) Institute of Molecular Pathology, Dr. Bohr Gasse 7, A-1030 Vienna, Austria, AT;(3) Departments of Ophthalmology and Visual Sciences and Molecular Genetics, Albert Einstein College of Medicine, 909 Ullmann, 1300 Morris Park Avenue, Bronx, NY 10461, USA, e-mail: cvekl@aecom.yu.edu, Tel.: +1-718-4303217, Fax: +1-718-4308778, US |
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Abstract: | Recent genetic analysis of the Drosophila dachshund (dac) gene has established that dac encodes a novel nuclear protein that is involved in both eye and leg development. In the Drosophila eye, dac expression appears to be controlled by the product of the eyeless/Pax6 gene. In order to analyze the Pax6 pathway in vertebrates we have isolated and characterized the cDNA and genomic clones corresponding to the human and mouse
homologues of Drosophila
dac. A full-length human cDNA encoding dachshund (DACH) encodes the 706 amino acids protein with predicted molecular weight of 73 kDa. A 109 amino acid domain located at the N-terminus
of the DACH showed significant sequence and secondary structure homologies to the ski/sno oncogene products. Northern blot
analysis found human DACH predominantly in adult kidney, heart, and placenta, with less expression detected in the brain,
lung, skeletal muscle and pancreas. A panel of human cell lines was studied and most notably a large proportion of neuroblastomas
expressed DACH mRNA. Mouse Dach encodes a protein of 751 amino acids with predicted molecular weight of 78 kDa that is 95%
identical to the human DACH. RNase protection analysis showed the highest Dach mRNA expression in the adult mouse kidney and
lung, whereas lower expression was detected in the brain and testis. RT/PCR analysis readily detected Dach mRNA in the adult
mouse cornea and retina. Dach mRNA expression in the mouse E11.5 embryo was observed primarily in the fore and hind limbs,
as well as in the somites.
Received: 9 February 1999 / Accepted: 19 April 1999 |
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Keywords: | Dachshund Pax6 Eye Limb Mammalian development |
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