Structure and RNA content of the prosomes. |
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| Authors: | O Coux H G Nothwang K Scherrer W Bergsma-Schutter A C Arnberg P A Timmins J Langowski C Cohen-Addad |
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| Institution: | Institut Jacques Monod, Paris, France. |
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| Abstract: | Duck erythroblasts prosomes were analysed by small angle neutron scattering (SANS), dynamic light scattering and (cryo-)electron microscopy. A molecular weight of approximately 720,000 +/- 50,000, a radius of gyration of 64 +/- 2 A and a hydrodynamic radius of approximately 86 A were obtained. Electron micrographs show a hollow cylinder-like particle with a diameter of 120 A, a height of 170 A and a diameter of 40 A for the cavity, built of four discs, the two outer ones being more pronounced than those in the center. Results from SANS indicate less then 5% of RNA in the purified prosomes, but nuclease protection assays confirm its presence. |
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