Surface plasmon field-enhanced fluorescence spectroscopy apparatus with a convergent optical system for point-of-care testing |
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Authors: | Mitsuaki Toda Yusuke Arima Hiromi Takiguchi Hiroo Iwata |
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Institution: | 1. Institute for Frontier Medical Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8507, Japan;2. Graduate School of Engineering, University of Tokyo, Bunkyo-ku, Tokyo 113-8656, Japan |
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Abstract: | Surface plasmon field-enhanced fluorescence spectroscopy (SPFS) is a promising methodology for point-of-care (POC) testing. The SPFS devices that have been reported are equipped with an angle rotating stage to adjust the surface plasmon resonance (SPR) angle. In a clinical setting, however, the SPR angle determination is a tedious and time-consuming process. In this study, we employed an SPFS instrument with a convergent optical system that allows the omission of this procedure. We demonstrated that this instrumentation allowed the sensitive determination of low concentrations of α-fetoprotein in serum and reduced the variation effect caused by the protein concentrations in samples. The SPFS with a convergent optical system is suitable for POC testing. |
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Keywords: | Surface plasmon field-enhanced fluorescence spectroscopy Convergent optical system Tumor marker Highly sensitive detection |
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