Monitoring the progression of loop-mediated isothermal amplification using conductivity |
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| Authors: | Xuzhi Zhang Wenwen Liu Xun Lu J. Justin Gooding Qiufen Li Keming Qu |
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| Affiliation: | 1. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Qingdao 266071, People’s Republic of China;2. College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266100, People’s Republic of China;3. School of Chemistry and The Australian Centre for NanoMedicine, University of New South Wales, Sydney, NSW 2052, Australia |
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| Abstract: | Loop-mediated isothermal amplification (LAMP) yields a large amount of DNA, as well as magnesium pyrophosphate precipitate, causing a decrease in ionic strength that can be measured with a conductivity meter. There is a clear relationship between the conductivity of the LAMP mixture solution and the duration of biochemical reaction. Moreover, there is also a clear relationship between the change in conductivity and the amount of initial template DNA over the range of 0.08 to 3.2 ng. These results demonstrate the feasibility not only for detecting the LAMP product qualitatively but also for real-time monitoring the biochemical reaction progression quantitatively using conductivity measurements. |
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| Keywords: | Loop-mediated isothermal amplification Conductivity Monitor biochemical reaction Gene analysis method |
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