Two fluorogenic substrates for purine nucleoside phosphorylase,selective for mammalian and bacterial forms of the enzyme |
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Authors: | Jacek Wierzchowski Alicja Stachelska-Wierzchowska Beata Wielgus-Kutrowska Goran Mikleuševi? |
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Institution: | 1. Department of Biophysics, University of Varmia and Masuria, 10-719 Olsztyn, Poland;2. Division of Biophysics, Institute of Experimental Physics, University of Warsaw, 02-089 Warsaw, Poland;3. Division of Physical Chemistry, Rudjer Boškovi? Institute, HR-10002 Zagreb, Croatia |
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Abstract: | Two nontypical nucleosides, 7-β-d-ribosyl-2,6-diamino-8-azapurine and 8-β-d-ribosyl-2,6-diamino-8-azapurine, have been found to exhibit moderately good, and selective, substrate properties toward calf and bacterial (Escherichia coli) forms of purine nucleoside phosphorylase (PNP). The former compound is effectively phosphorolysed by calf PNP and the latter by PNP from E. coli. Both compounds are fluorescent with λmax ∼ 425 to 430 nm, but the reaction product, 2,6-diamino-8-azapurine, emits in a different spectral region (λmax ∼ 363 nm) with nearly 40% yield, providing a strong fluorogenic effect at 350 to 360 nm. |
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Keywords: | Fluorogenic substrates 8-Azapurines Nucleoside analogs Purine nucleoside phosphorylase |
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