Dual genetically encoded phage-displayed ligands |
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| Authors: | Kritika Mohan Gregory A. Weiss |
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| Affiliation: | 1. Department of Chemistry, University of California, Irvine, Irvine, CA 92697, USA;2. Department of Molecular Biology and Biochemistry, University of California, Irvine, Irvine, CA 92697, USA |
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| Abstract: | M13 bacteriophage display presents polypeptides as fusions to phage coat proteins. Such phage-displayed ligands offer useful reagents for biosensors. Here, we report a modified phage propagation protocol for the consistent and robust display of two different genetically encoded ligands on the major coat protein, P8. The results demonstrate that the phage surface reaches a saturation point for maximum peptide display. |
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| Keywords: | Bacterial transformation Phage Phage display ELISA |
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