A colorimetric sandwich-type assay for sensitive thrombin detection based on enzyme-linked aptamer assay |
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Authors: | Jun Hee Park Yea Seul Cho Sungmuk Kang Eun Jeong LeeGwan-Ho Lee Sang Soo Hah |
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Institution: | Department of Chemistry and Research Institute for Basic Sciences, Kyung Hee University, Seoul 130-701, Republic of Korea |
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Abstract: | A colorimetric sandwich-type assay based on enzyme-linked aptamer assay has been developed for the fast and sensitive detection of as low as 25 fM of thrombin with high linearity. Aptamer-immobilized glass was used to capture the target analyte, whereas a second aptamer, functionalized with horseradish peroxidase (HRP), was employed for the conventional 3,5,3′,5′-tetramethylbenzidine (TMB)-based colorimetric detection. Without the troublesome antibody requirement of the conventional enzyme-linked immunosorbent assay (ELISA), as low as 25 fM of thrombin could be rapidly and reproducibly detected. This assay has superior, or at least equal, recovery and accuracy to that of conventional antibody-based ELISA. |
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Keywords: | Aptamer Thrombin Signal amplification ELISA |
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