A chromogenic assay for limit dextrinase and pullulanase activity |
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Authors: | Marie Bøjstrup Caspar Elo Christensen Michael Skovbo WindahlAnette Henriksen Ole Hindsgaul |
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Institution: | Carlsberg Laboratory, Gamle Carlsberg vej 10, 1799 Copenhagen V, Denmark |
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Abstract: | A new chromogenic substrate to assay the starch debranching enzymes limit dextrinase and pullulanase is described. The 2-chloro-4-nitrophenyl glycoside of a commercially available branched heptasaccharide (Glc-maltotriosyl-maltotriose) was found to be a suitable specific substrate for starch debranching enzymes and allows convenient assays of enzymatic activities in a format suited for high-throughput analysis. The kinetic parameters of these enzymes toward the synthesized substrate are determined, and the selectivity of the substrate in a complex cereal-based extract is established. |
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Keywords: | Coupled assay Limit dextrinase Pullulanase Chromogenic substrate |
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