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Uptake and aminoacylation of exogenous transfer RNA by mouse leukemia cells
Authors:R E Gallagher  C A Walter  R C Gallo
Affiliation:1. State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China;2. Department of Pharmacology, School of Pharmacy, China Pharmaceutical University, Nanjing 210009, China;3. Key Laboratory of RNA Science and Engineering, State Key Laboratory of Molecular Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai 200031, China;4. State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China;5. Department of Biochemistry, School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, China;6. Chongqing Innovation Institute of China Pharmaceutical University, Chongqing 401135, China;7. Key Laboratory of Systems Health Science of Zhejiang Province, School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou 310024, China
Abstract:Effective uptake of exogenous 3H-labelled tRNA into the active metabolic tRNA pool of mouse L1210 cells was demonstrated by detecting aminoacyl-(3H) tRNA synthesized in the intact, pulsed cells.
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