| 神经细胞老化实验研究模型:无血清条件下培养小鼠神经母细胞瘤细胞 |
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| 引用本文： | 蔡琰,沈金坤,陆荣华. 神经细胞老化实验研究模型:无血清条件下培养小鼠神经母细胞瘤细胞[J]. 分子细胞生物学报, 1985, 0(4) |
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| 作者姓名： | 蔡琰 沈金坤 陆荣华 |
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| 作者单位： | 上海第二医学院附属仁济医院神经生物学实验室(蔡琰，沈金坤)，中国科学院上海细胞生物学研究所(陆荣华) |
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| 摘 要： | 本文报道的在无血清条件下培养小鼠神经母细胞瘤细胞,应用显微荧光光度测定法来定量观察脂褐素自发荧光现象的增长,是神经细胞老化实验研究一种实用的模型,可适用于多种基础或应用的研究。我们认为对某些细胞株,在适用的实验研究情况下,应用较简单的无血清培养条件完全是可以实行的,应该提倡推广。
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AN EXPERIMENTAL MODEL FOR THE STUDY OF AGING OF NEURONS:SERUM-FREE CULTURE OF MOUSE NEUROBLASTOMA CELLS |
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| TSAI YEN SHEN JIN-KUN Lu JUNG-HUA. AN EXPERIMENTAL MODEL FOR THE STUDY OF AGING OF NEURONS:SERUM-FREE CULTURE OF MOUSE NEUROBLASTOMA CELLS[J]. Journal of Molecular Cell Biology, 1985, 0(4) |
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| Authors: | TSAI YEN SHEN JIN-KUN Lu JUNG-HUA |
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| Abstract: | We report a practical model for thestudy of aging of neurons.Thisconsisted of a simple serum-free cultureof mouse neuroblastoma cells with albu-min,insulin and DMEM.Microfluo-rometry of the autofluorescence of lipo-fuscin was utilized to provide quanti-tative data about the increasing accumu-lation of lipofuscin with time.Thismodel could serve both basic andapplied research purposes. |
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| Keywords: | Neuroblastoma cells.Serum-free culture.Lipofuscin microfluorometry. |
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