| Abstract: | This is the first report demonstrating hormonal control of cAMP levels in Ewing's sarcoma cells. Two classes of hormones, beta-adrenergic agonists and prostaglandins stimulate cAMP production in cultured Ewing's sarcoma cells. The efficacy order for beta-adrenergic agonists is (-)-isoprenaline greater than or equal to (-)-noradrenaline greater than or equal to (-)-adrenaline much greater than (-)-phenylephrine. The stimulatory effect of (-)-noradrenaline is antagonized by beta 1-selective metoprolol and also by beta 2-selective ICI 118,551. The efficacy order for prostaglandins (PG) is PGE1 greater than PGI2 greater than PGE2 much greater than PGF2 alpha; 6-keto PGF1 alpha and PGD2 do not influence cAMP levels in Ewing's sarcoma cells. Cultures pretreated with PGE2 are less responsive to a second challenge with PGE2 but their response to (-)-isoprenaline is unimpaired. Similarly, pretreatment with (-)-isoprenaline induces homologous desensitization to (-)-isoprenaline and has no effect on the PGE2-stimulated increase in cAMP. We conclude that these cells provide an ideal model for the study of the initial steps of beta-adrenergic and prostaglandin action in Ewing's sarcoma. |
