Detection of RNA on northern blots by negative staining with aurintricarboxylic acid. |
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Authors: | I Oberb?umer C Speth |
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Institution: | Max-Planck-Institut für Biochemie, Martinsried, Federal Republic of Germany. |
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Abstract: | Aurintricarboxylic acid (ATA) is a well-known inhibitor of RNA and DNA modifying enzymes and was suggested as a potent RNase inhibitor for preparation of RNA (Hallick et al., 1977, Nucleic Acids Res. 4, 3055-3064). We show that ATA is a very useful stain for detecting RNA on Northern blots and slot blots although it did not fully protect purified RNA in concentrated solution against RNase A. |
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