Autologous and homologous immunofluorescent antibody to established breast cancer cell lines |
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Authors: | Charles Wiseman Relda Cailleau Matilde Olivé George R Blumenschein James M Bowen |
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Institution: | (1) Department of Medicine, The University of Texas System Cancer Center, M. D. Anderson Hospital and Tumor Institute, Texas Medical Center, 77030 Houston, Texas;(2) Department of Molecular Carcinogenesis and Virology, The University of Texas System Cancer Center, M. D. Anderson Hospital and Tumor Institute, Texas Medical Center, 77030 Houston, Texas |
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Abstract: | Summary Indirect immunofluorescence tests were performed on 14 established human breast cancer cell lines using sera from a variety
of subjects. Autologous reactions were studied on 10 cell lines, with positive reactions demonstrable in 8. Tests using sera
from a randomly selected population of breast cancer patients showed reactivity in 40 to 66% depending on the target cell
line used. Reactivity to other nonbreast cancer cell lines was rare. Several control populations were tested, including normal
blood bank donors, persons with benign breast disease, and persons with other forms of cancer; immunofluorescent antibody
was detected much less frequently in sera from these populations than those from the breast cancer group. Positive reactions
remained in spite of absorption of serum with heterophile antigens, normal human breast tissue, and AB+ red blood cells. Thus
established cell lines of human breast cancer possess antigens commonly recognized by sera from breast cancer patients.
This work was supported in part by Grant CA 16789 from the National Cancer Institute, NIH, Department of Health, Education
and Welfare. |
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Keywords: | breast cancer cell lines immunofluorescent antibody |
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