| PCR法检测对虾皮下和造血器官坏死杆状病毒 |
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| 引用本文： | 夏春,黄捷.PCR法检测对虾皮下和造血器官坏死杆状病毒[J].微生物学报,1999,39(2):171-173. |
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| 作者姓名： | 夏春 黄捷 |
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| 作者单位： | 中国农业大学动物医学院 北京 100094；中国水产科学研究院黄海水产研究所 青岛 266071 |
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| 基金项目： | 国家教委回国人员启动资金 |
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| 摘 要： | 皮下和造血器官坏死杆状病毒（ＨＨＮＢＶ）［１］、对虾杆状ＤＮＡ病毒（ＰＲＤＶ）［２］和白斑杆状病毒（ＷＳＢＶ）［３］是近年来引起全球对虾暴发性死亡的病原。这三种病毒同属杆状病毒属Ｃ杆状病毒亚群中的新病毒，在流行病学和病理学特征上十分类似，因此，我们...
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| 关 键 词： | PCR法，皮下和造血器官坏死杆状病毒，对虾，暴发性流行病 |
DETECTION OF HYPODERMAL AND HEMATOPOIETIC NECROSIS BACULOVIRUS BY PCR METHOD |
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| Xia Chun.DETECTION OF HYPODERMAL AND HEMATOPOIETIC NECROSIS BACULOVIRUS BY PCR METHOD[J].Acta Microbiologica Sinica,1999,39(2):171-173. |
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| Authors: | Xia Chun |
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| Institution: | Department to Microbiology an Infection, College of Velerinary Medicine, China Agricultural University Beijing 100094. |
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| Abstract: | For control of the explosive epidemic disease of Penaeid shrimp in China. We have developed a method for detection of Hypodermal and hematopoietic necrosis baculovirus (HHNBV) by polymerase chain reaction (PCR). Two pairs of PCR primers (DP1/DP2 and DP3/DP4) were prepared, based on the RFLP analysis between HHNBV and penaeid rod shaped DNA virus. PCR using either DP1/DP2 or DP3/DP4 specified HHNBV genome. When PCR products amplified with DP1/DP2 were subjected to nested PCR with DP3/DP4, a high sensitivity about 0.04pg was seem with the nested PCR. |
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| Keywords: | PCR Hypodermal and hematopoietic necrosis baculovirus Penaeid shrimp Explosive epidemic disease |
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