| 马立克氏病病毒REispens株B抗原基因的克隆及其重组鸡痘病毒的构建 |
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| 引用本文： | 邢力 彭大新. 马立克氏病病毒REispens株B抗原基因的克隆及其重组鸡痘病毒的构建[J]. 微生物学报, 1999, 39(2): 164-167 |
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| 作者姓名： | 邢力 彭大新 |
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| 摘 要： |
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| 关 键 词： | 马立克氏病病毒 糖蛋白B抗原 重组鸡痘病毒 |
Cloning of glycoprotein B gene from strain Rispens of Marek's disease virus and construction of recombinant fowlpox virus] |
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| L Xing,D Peng,A Zhu,X Liu,R Zhang. Cloning of glycoprotein B gene from strain Rispens of Marek's disease virus and construction of recombinant fowlpox virus][J]. Acta microbiologica Sinica, 1999, 39(2): 164-167 |
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| Authors: | L Xing D Peng A Zhu X Liu R Zhang |
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| Affiliation: | Department of Veterinary Medicine, Agricultural College, Yangzhou University, Yangzhou 225009. |
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| Abstract: | Purified DNAs from Chicken Embryo Fibroblast (CEF) cultures infected with MDV strain Rispens were used as templates. Specific fragment with the size of about 2.9 kb was successfully amplified through Polymerase Chain Reaction(PCR) and identified to be gB gene of MDV by dot blot hybridization with a digoxigenin-labelled MDV gB specific oligonucleotide probe. The gB gene from strain Rispens was cloned into pUC19 and FPV insertion vector pFG1175-1 to construct plasmid pMGB and pFGBR1775-1 respectively. DOSPER liposome-mediated transfection with insertion vector DNA pFGBR1175-1 was performed on CEF monolayers infected with FPV 3-4 h earlier. Recombinant FPV was clone purified. Immunofluorescence Assay(IFA) showed that MDV gB gene had been expressed in FPV. |
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