食酸丛毛单胞菌AN3菌株降解苯胺代谢途径的研究

食酸丛毛单胞菌(\%Comamonas acidovorans\%)AN3菌株中降解苯胺的酶类均为诱导酶，在以苯胺为唯一碳、氮源和能源生长的细胞中，含有苯胺双加氧酶、邻苯二酚2，3双加氧酶、2羟基己二烯半醛酸脱氢酶、4草酰巴豆酸脱羧酶和4羟基2酮戊酸醛缩酶等。苯胺双加氧酶作用于苯胺的\%K\%m值和\%V\%\-\{max\}分别为292μmol/L和3.57μmol\5mg\+\{-1\}·min-1;邻苯二酚2,3双加氧酶作用于邻苯二酚的\%K\%m和\%V\%\-\{max\}分别为16.4 mol/L和15.2μmol\5mg\+\{-1\}\5min\+\{-1\}。根据实验结果，推测了该菌株降解苯胺的代谢途径。

STUDIES ON THE METABOLISM PATHWAY OF ANILINE DEGRADATION BY COMAMONAS ACIDOVORANS AN3

All of the enzymes relating to aniline degradation in C. acidovorans AN3 were inducible ones. When growing on aniline as sole carbon, nitrogen and energy sources, the cells of C. acidovorans AN3 contained aniline dioxygenase, catechol-2, 3-dioxygenase, 2-hydromuconic semialdehyde dehydrogenase, 4-oxalocrotonic acid decarboxylase and 4-hydroxy-2-oxovalerate acid aldolase. The Km and Vmax of aniline dioxygenase were 292 mumol/L and 3.57 mumol.mg-1. min-1, respectively. The Km and Vmax of catechol-2, 3-dioxygenase were 16.4 mumol/L and 15.2 mumol.mg-1.min-1, respectively. According to these results, the degradation metabolism pathway of aniline by C. acidovorans AN3 was hypothesised.