| 抗真菌肽LP-1的分离纯化及特性分析 |
| |
| 引用本文： | 刘颖 徐庆 陈章良. 抗真菌肽LP-1的分离纯化及特性分析[J]. 微生物学报, 1999, 39(5): 441-447 |
| |
| 作者姓名： | 刘颖 徐庆 陈章良 |
| |
| 作者单位： | 北京大学生命科学院蛋白质工程及植物基因工程国家重点实验室 北京 100871 |
| |
| 摘 要： | 拮抗菌枯草芽孢杆菌(%Bacillus subtilis)%TG|26分泌产生的小肽经两次盐酸沉淀、丙酮分级沉淀和Hi|pore反相柱两次纯化，分离得到一种新的抗真菌的小肽，命名为LP|1。经MALDI|TOF质谱鉴定，分子 量为10573,等电聚焦测得其pI为475。LP|1对温度有较高的稳定性，100℃保温30min,仍能保持75%的活性。抑菌谱表明，该抗菌肽对瓜果腐霉%(Pythium aphanidermatum),玉蜀黍赤霉病菌(Gibberella zeae),长柄链格孢(Alternaria longipe)和番茄蔫萎座镰孢霉(Fusarium oxysporum %f.%lycopersici)等植物病原真菌有很强的抑制作用。LP1可造成绿色木霉(Trichoderma viride)%菌丝生长形态异常：菌丝端部膨大，菌丝扭曲，分支加剧，菌丝内细胞质分布不均匀，发生凝聚。茚三酮反应以及测序结果均证实其为环肽。
|
| 关 键 词： | 枯草芽孢杆菌TG26，抗菌肽，抑菌谱，环肽 |
PURIFICATION AND CHARACTERIZATION OF ANTIFUNGAL PEPTIDELP 1 |
| |
| Liu Ying Xu Qing Chen Zhangliang. PURIFICATION AND CHARACTERIZATION OF ANTIFUNGAL PEPTIDELP 1[J]. Acta microbiologica Sinica, 1999, 39(5): 441-447 |
| |
| Authors: | Liu Ying Xu Qing Chen Zhangliang |
| |
| Affiliation: | National Laboratory of Protein Engineering and Plant Genetic Engineering, Peking University, Beijing 100871. |
| |
| Abstract: | An antifungalpeptide LP 1 from Bacillussubtilis TG26 strain was purified by acid pre cipitation,acetone precipitation and Hi porereversed phase column chromatography.The molecular weightof LP 1is1057.3 Dasdetermined by MALDI TOFmassspectrometry,andits pIis 4 .75 by PAG IEF.It wasalsofound to bethermostable.Its antifungalspectrum showed that LP 1 has stronginhibitory activity against many plant pathogenicfungi,such as Pythium aphanidermatum ,Gibberella zeae,Alternaria longipe,Fusarium oxysporum f.lycopersici,etc.. The abnormal hy phalgrowth of Trichoderma viridecaused by LP 1 such asswollentips,twisted,shortgrowth and cytoplasm condensation wasalsoobserved.Both ninhydrinreaction and peptidesequencingsuggested that LP 1is acyclic peptide. |
| |
| Keywords: | Bacillussubtilis TG 26 Antifungalpeptide Antifungalspectrum Cyclic peptide |
| 本文献已被 CNKI 维普 等数据库收录！ |
| 点击此处可从《微生物学报》浏览原始摘要信息 |
|
点击此处可从《微生物学报》下载全文 |
