| 土壤微生物PCR及分子杂交检测 |
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| 引用本文： | 丁志勇 许崇任. 土壤微生物PCR及分子杂交检测[J]. 微生物学报, 1999, 39(4): 381-384 |
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| 作者姓名： | 丁志勇 许崇任 |
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| 作者单位： | 北京大学生命科学学院环境生物学及生态学系 |
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| 摘 要： | ＰＣＲ技术在环境微生物的检测方面已得到越来越广泛的应用，Ｓｔｅｆａｎ等［１］利用ＰＣＲ技术检测土壤中的转基因细菌，后来用于检测土壤中不可培养的微生物［２］，跟踪环境中的特定细菌或ＤＮＡ［３］，揭示土壤生态系统的基因多样性［４］等。利用ＰＣＲ技术来检测…
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| 关 键 词： | PCR 分子杂交 苏云金芽孢杆菌 检测 |
DETECTING MICROORGANISMS IN SOIL BY PCR AND MOLECULAR HYBRIDIZATION |
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| Ding Zhiyong Xu Chongren. DETECTING MICROORGANISMS IN SOIL BY PCR AND MOLECULAR HYBRIDIZATION[J]. Acta microbiologica Sinica, 1999, 39(4): 381-384 |
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| Authors: | Ding Zhiyong Xu Chongren |
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| Affiliation: | Environmental Biology and Ecology Department, College of Life Sciences, Peking University, Beijing 100871. |
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| Abstract: | PCR and molecular hybridization provide new methods to detect microorganisms in environment. A simple, sensitive protocol was developed to detect Bacillus thuringiensis HD-1 in soil by PCR and Southern Blotting in this study. Methods of extracting DNA from soil and purification were compared. By a modified total DNA extraction from soil and purification, followed by PCR and Southern Blotting, strain HD-1 released into soil can be detected with a sensitivity of 2.2 x 10(2) cells/g soil. By using other specific primers, this detection protocol can be widely used in the research of ecology and risk assessment of genetically engineered microorganisms released into environment. |
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| Keywords: | PCR Molecular hybridization Bacillus thuringiensi s Detection |
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