| 链霉菌质粒pSGL1最小复制子序列测定及分析 |
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| 引用本文： | 张华,洪斌,李元.链霉菌质粒pSGL1最小复制子序列测定及分析[J].微生物学报,1999,39(4):327-332. |
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| 作者姓名： | 张华 洪斌 李元 |
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| 作者单位： | 中国医学科学院协和医科大学医药生物技术研究所 |
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| 摘 要： | 质粒ｐＳＧＬ１（７．４ｋｂ）是从球孢链霉菌（Ｓｔｒｅｐｔｏｍｙｃｅｓｇｌｏｂｉｓｐｏｒｕｓ）中分离得到的一个高拷贝质粒，已证明其最小复制子位于Ｓａｕ３ＡＩ酶切的２０ｋｂ片段上。对该片段进行亚克隆，测序后数据表明该片段是一个新序列。仅有一个开放阅读框架（ＯＲＦＲ）位于最小复制子中，推测其编码的蛋白质含有滚环复制质粒复制酶的特定序列。
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| 关 键 词： | 质粒 最小复制子 滚环复制 复制酶 |
NUCLEOTIDE SEQUENCE ANALYSIS OF THE MINIMAL REPLICON OF THE STREPTOMYCES PLASMID pSGL1 |
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| Zhang Hua,Hong Bin,Li Yuan.NUCLEOTIDE SEQUENCE ANALYSIS OF THE MINIMAL REPLICON OF THE STREPTOMYCES PLASMID pSGL1[J].Acta Microbiologica Sinica,1999,39(4):327-332. |
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| Authors: | Zhang Hua Hong Bin Li Yuan |
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| Institution: | Institute of Medicinal Biotechnology, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100050. |
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| Abstract: | The high copy number plasmid pSGL1(7.4kb) was isolated from Streptomyces globisporus . Deletion experiments showed its minimal replicon is located on the 2.0kb Sau 3 AI fragment. This fragment was subcloned. DNA sequence data analysis showed this fragment is a new sequence. Only an open reading frame with high coding probability is located on the minimal replicon. The deduced protein contains motifs characteristic of replicase for rolling circle replication. |
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| Keywords: | Plasmid Minimal replicon Rolling circle replication Replicase |
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