EB病毒潜伏膜蛋白1基因对上皮细胞增殖的影响

为了研究ＥＢ病毒潜伏膜蛋白１（ＬＭＰ１）基因对上皮细胞增殖的影响，探索ＬＭＰ１在上皮细胞肿瘤发生中所起的作用。用ＬＭＰ１基因真核表达质粒转染人胚肾上皮细胞，检测了转染细胞中ＬＭＰ１的表达，观察细胞在软琼脂中的集落形成能力、ＭＴＴ吸收能力以及ＰＣＮＡ的表达情况。结果显示，被ＬＭＰ１基因转染的细胞生长旺盛，能在软琼脂中形成多个集落，ＭＴＴ吸收能力增强，ＰＣＮＡ的表达水平增高。因此认为ＬＭＰ１基因能明显改变上皮细胞的生物学行为，促进细胞的生长、增殖和转化，使转染的上皮细胞获得肿瘤细胞的生长特征

Influence of EBV latent membrane protein 1 gene on the proliferation of epithelial cells]

In order to study the influence of EBV latent membrane protein (LMP1) gene on the proliferation of epithelial cells and the role of LMP1 creating to epithelial cell carcinomas, EBV LMP1 gene eukaryotic expression plasmid was transfected into human embryo kidney epithelial cells. The expression of LMP1 protein in transfected cells was detected. Observing the formation of colonies in soft agar, the value of MTT absorbed and the expression of PCNA, the results showed that the transfected cells grew vigorously and formed more colonies in soft agar, the MTT uptake and PCNA expression were apparently increased. The conclusion was that LMP1 gene could alter the biological behaviours of epithelial cells, promote cell growth, proliferation and transformation and endowed the transfected cells with growth characteristics like tumor cells.