基因型和培养条件对大麦花药培养的影响

不同基因型大麦(Hordeum vulgare)的花粉愈伤组织诱导率不同。带大麦黄花叶病抗性基因的品种。品系或F1杂种的诱导率(8.83—14.21%)高于不带抗性基因的材料(1.04—6.25%)。 MS基本培养基添加草2，4—D.1 mg/L，Kt0.1 mg／L，BA0.2mg／L，生物素0.1mg, 其诱导率(平均11.09%)高于MS添加2，4—D3mg／L和Kt0.1 mg／L的诱导率(6.33%)及MS添加2，4—D1mg／L 和Kt 0.1 mg／L的诱导率(8.21%)。接种后先15℃暗培养5天，再转入25℃暗培养，其产生愈仿组织的高峰期推迟2—4天，但诱导率却从对照的5.84%提高到11.59%；25℃暗培养与25℃光 — 暗交替培养，诱导率无显著差异。

Effects of Genotype and Culture Condition on Anther Culture in Barley(Hordeum Vulgare)

There was a wide range of variation (from 1.04% to 14.21%) among 12 genotypes for callus induction frequency, although all of them formed calli. Cultivars, lines and F1 hybrids carring BYMV resistance genes had higher callus induction frequency (8.83%-14.21%) than that of others (1.04%-6.25%). The comparison of callus induction frequency in four media revealed that when the medium was supplemented with 1.0 mg/L 2, d-D, 0.1 mg/L Kt, 0.2 mg/L BA and 0.1 mg/L Biotin, it was more suitable (ll.09%)than the other three media (6.33 %, 8.21% and 10.82% respectively). The results indicated that after incubating the anther at 15℃ for 5 days, then incubate them at 25℃ in the dark(condition 1), increased the callus induction frequency (from 5.84% to 11.59%), although the peak of the callus induction appeared 2-4 days later. No statistical differences were found for the callus induction frequency between dark culture (condition 2) and light/dark culture (condition 3).