In vivo iron and zinc deficiency diminished T- and B-selective mitogen stimulation of murine lymphoid cells through protein kinase C-mediated mechanism |
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Authors: | A J Klecha J Salgueiro M Wald J Boccio M Zubillaga N M Leonardi G Gorelik G A Cremaschi |
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Institution: | (1) Laboratorio de Radioisótopos, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina;(2) Centro de Estudios Farmacológicos y Botánicos (CEFYBO), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina |
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Abstract: | Zinc and iron are crucial mineral components of human diet, because their deficiency leads to several disorders, including
alterations of the immune function. It has been demonstrated, in both humans and rodents, that a diminished number of lymphoid
cells and a loss of lymphocyte activity accompany deprivation of these essential minerals. The aim of this work was to analyze
if iron and/or zinc imbalances regulate lymphocyte activity and the intracellular signals involved in the effect. Mice from
the BALB/c strain were fed with iron- and/or zinc-deficient or mineral-supplemented diets, according to the American Institute
of Nutrition Rodent Diets. Levels of iron and zinc were assessed in blood, liver, or bone samples. Selective mitogen stimulation
of T- and B-lymphocytes were performed. We found a diminished proliferative response in T- and B-lymphocytes from zinc- and/or
iron-deficient animals with respect to controls. These effects were related to decreased mitogen-induced translocation of
protein kinase C (PKC) activity to cell membranes on both cell types from all animals fed with deficient diets. Our results
demonstrate that iron and zinc deficiencies affect both T- and B-lymphocyte function by PKC-dependent mechanisms. |
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Keywords: | Iron zinc T-lymphocyte B-lymphocyte proliferation protein kinase C |
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