Heterogeneous set of cell wall teichoic acids in strains of <Emphasis Type="Italic">Bacillus subtilis</Emphasis> VKM B-760 and VKM B-764 |
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Authors: | N V Potekhina G M Streshinskaya Yu I Kozlova E B Kudryashova S N Senchenkova A S Shashkov L N Anan’ina |
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Institution: | 1.Faculty of Biology,Lomonosov Moscow State University,Moscow,Russia;2.All-Russian Collection of Microorganisms, Skryabin Institute of Biochemistry and Physiology of Microorganisms,Russian Academy of Sciences,Pushchino, Moscow Region,Russia;3.Zelinsky Institute of Organic Chemistry,Russian Academy of Sciences,Moscow,Russia;4.Institute of Ecology and Genetics of Microorganisms,Ural Branch of the Russian Academy of Sciences,Perm’,Russia |
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Abstract: | Cell walls of Bacillus subtilis VKM B-760 and VKM B-764 are characterized by heterogeneous composition of teichoic acids. Polymer I with structure -6)-β-D-Galp-(1→1)-sn-Gro-(3-P-, polymer II with structure -6)-α-D-Glcp-(1→1)-sn-Gro-(3-P-, and a small amount of unsubstituted 1,3-poly(glycerol phosphate) were detected in strain VKM B-760. Strain VKM
B-764 contains an analogous set of teichoic acids, but a characteristic feature of polymer II is the presence of disubstituted
glycerol residue with α-glucopyranose localization in the integral chain at C-1 hydroxyl and β-glucopyranose as a side branch
at C-2 hydroxyl (polymer III): -6)-α-D-Glcp-(1→1)-β-D-Glcp-(1→2)]-sn-Gro-(3-P-. The structures of polymer I in bacilli and polymer III in Gram-positive bacteria are described for the first time.
Teichoic acids were studied by chemical methods and on the basis of combined analysis of one-dimensional 1H-, 13C-, and 31P-NMR spectra, homonuclear two-dimensional 1H/1H COSY, TOCSY, and ROESY, and heteronuclear two-dimensional 1H/13C gHSQC- and HMQC-TOCSY experiments. Simultaneous presence of several different structure teichoic acids in the bacillus cell
walls as well as chemotaxonomical perspectives of the application of these polymers as species-specific markers for members
of the Bacillus genus is discussed. |
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