| 甘蓝夜蛾两种不同类型几丁质脱乙酰基酶基因的克隆与组织特异性表达 |
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| 引用本文： | 姚磊,樊东,王晓云,高艳玲. 甘蓝夜蛾两种不同类型几丁质脱乙酰基酶基因的克隆与组织特异性表达[J]. 昆虫知识, 2011, 48(5): 1417-1424 |
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| 作者姓名： | 姚磊 樊东 王晓云 高艳玲 |
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| 作者单位： | 东北农业大学农学院 哈尔滨150030 |
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| 基金项目： | 黑龙江省博士后科研启动金(LBH-Q09171); 哈尔滨市科技创新人才研究专项资金项目(RC2006QN002027) |
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| 摘 要： | 几丁质脱乙酰基酶(chitin deacetylase,CDA)是昆虫几丁质降解酶中的一种酶,可以将几丁质转化为壳聚糖,在昆虫几丁质代谢中具有重要作用.本研究以甘蓝夜蛾Mamestra brassicae5龄幼虫虫体为材料提取总RNA,利用RT-PCR和RACE技术,分别扩增得到甘蓝夜蛾的2类不同几丁质脱乙酰基酶基因的...
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| 关 键 词： | 甘蓝夜蛾 几丁质脱乙酰基酶 克隆 序列分析 组织表达 |
Molecular cloning and tissue-specific expression of two different chitin deacetylase cDNA sequences from Mamestra brassicae |
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| YAO Lei,FAN Dong,WANG Xiao-Yun,GAO Yan-Ling. Molecular cloning and tissue-specific expression of two different chitin deacetylase cDNA sequences from Mamestra brassicae[J]. Entomological Knowledge, 2011, 48(5): 1417-1424 |
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| Authors: | YAO Lei FAN Dong WANG Xiao-Yun GAO Yan-Ling |
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| Affiliation: | YAO Lei** FAN Dong*** WANG Xiao-Yun GAO Yan-Ling(Northeast Agricultural University,Harbin 150030,China) |
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| Abstract: | Chitin deacetylase(CDA) is a kind of chitin-degrading enzyme found in insects that can turn chitin into chitosan and plays an important role in insects' chitin metabolism.Total RNA was isolated from the fifth instar larvae of Mamestra brassicae.Two CDA cDNA sequences were cloned by RT-PCR and rapid amplification of cDNA ends(RACE).One cDNA sequence was 2 036 base pairs in length and contained an open reading frame of 1 617 base pairs coding for a polypeptide of 539 amino acid residues with a predicted molec... |
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| Keywords: | Mamestra brassicae chitin deacetylase cloning sequence analysis tissue-specific expression |
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