| 一种褐飞虱病原真菌的分子生物学鉴定 |
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| 引用本文： | 林华峰,李茂业,张松影,李世广,张承启. 一种褐飞虱病原真菌的分子生物学鉴定[J]. 昆虫知识, 2011, 48(5): 1401-1406 |
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| 作者姓名： | 林华峰 李茂业 张松影 李世广 张承启 |
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| 作者单位： | 安徽农业大学植物保护学院 合肥230036 |
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| 基金项目： | 公益性行业(农业)科研专项(200803003) |
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| 摘 要： | 从田间褐飞虱Nilaparvata lugens( St(a)l)罹病虫体分离得到一株绿僵菌,以该菌为供试菌体,采用氯化苄法、CTAB法以及裂解液法分别提取了该菌的基因组DNA；以ITS1和ITS4为绿僵菌通用引物,对供试菌株的rDNA ITS序列进行PCR扩增、琼脂糖凝胶电泳检测和序列分析,并在核酸序列数据库中进行同...
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| 关 键 词： | 绿僵菌 DNA提取 分子鉴定 |
Molecular biological identification of entomogenous fungi isolated from the brown planthopper |
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| LIN Hua-Feng,LI Mao-Ye,ZHANG Song-Ying,LI Shi-Guang,ZHANG Cheng-Qi. Molecular biological identification of entomogenous fungi isolated from the brown planthopper[J]. Entomological Knowledge, 2011, 48(5): 1401-1406 |
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| Authors: | LIN Hua-Feng LI Mao-Ye ZHANG Song-Ying LI Shi-Guang ZHANG Cheng-Qi |
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| Affiliation: | LIN Hua-Feng** LI Mao-Ye ZHANG Song-Ying LI Shi-Guang ZHANG Cheng-Qi(School of Plant Protection,Anhui Agricultural University,Hefei 230036,China) |
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| Abstract: | Three different methods were used to extract genomic DNA from a strain of Metarhizium spp.isolated from the surface of infected Nilaparvata lugens（Stl） collected from rice fields;benzyl chloride,CTAB and cracking solution.The fungus＇ rDNA ITS sequence was amplified with the primers ITS1 and ITS4,and the amplified ITS sequence detected by agarose gel electrophoresis and analyzed in Genebank.The results show that the cracking solution method obtained the highest quantity of the purest DNA,the benzyl chloride method was the next best and the CTAB method was not suitable.Homologous sequence contrast conducted in the DNA sequence database identified the fungus as Metarhizium flavoviride.Identifying the entomogenous fungi that infect N.lugens is an important first step in determining their control potential with respect to this pest. |
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| Keywords: | Metarhizium spp. DNA extraction molecular identification |
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