肚倍蚜对高单宁环境的抗氧化反应研究

肚倍蚜Kaburagia rhusicola Takagi是我国的一种重要的资源昆虫,其瘿内生活环境较特殊(虫瘿单宁含量为70%)。为了解肚倍蚜适应高单宁环境生化机制,本研究以性母为对照,测定了瘿内不同时期肚倍蚜体内总抗氧化能力(T-AOC)以及超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)和谷胱甘肽S-转移酶(GST)的活性,分析了肚倍蚜体内的抗氧化系统对高单宁环境胁迫所作出的相关反应。研究表明,与对照相比,瘿内时期肚倍蚜体内的总抗氧化能力、CAT和POD活性均显著升高;SOD和GST活性除在有翅蚜阶段与对照无差异,在其他阶段的活性均显著高于对照。本研究验证了高单宁环境对肚倍蚜造成氧化胁迫作用,认为肚倍蚜体内的抗氧化能力和抗氧化酶活性提高是其对高单宁环境的一种适应。

Research on the antioxidant response of Kaburagia rhusicola in high tannin environment

Kaburagia rhusicola Takagi（Hemiptera：Pemphigidae） is an economically important insect in China that lives in a special environment—galls with a high（70%） tannin content.In order to explore the biochemical mechanisms by which these aphids have adapted to tolerate tannin,we treated the sexupara as the control and measured the total antioxidant capacity and antioxidant enzymes activities of SOD,CAT,POD and GST in adult K.rhusicola of different generations.We found that,with the exception of the alate virginoparae generation,activities of SOD and GST in aphid generations living within galls were higher than in the control,and that T-AOC and POD activities increased over the course of the galls’ life.Our results confirm that a high tannin environment increases the level of oxidative stress on K.rhusicola and suggests that antioxidant activity may be an adaptation of aphids to the high-tannin gall environment.