| 小麦种子中几丁质酶基因的扩增及序列分析 |
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| 引用本文： | 易图永,池田逹哉. 小麦种子中几丁质酶基因的扩增及序列分析[J]. 生物信息学, 2007, 5(1): 1-4 |
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| 作者姓名： | 易图永 池田逹哉 |
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| 作者单位： | 1. 湖南农业大学生物安全科技学院,湖南,长沙,410128
2. 日本西部农业研究中心育种工学研究室,广岛,福山,062-8555 |
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| 基金项目： | 湖南省教育厅日元贷款项目 |
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| 摘 要： | 根据http://www.tigr.org中与小麦几丁质酶基因相关的序列TC187877,设计引物,分别从小麦品种Gamenya和苏麦3号中扩增到大小约为1 000bp的片段。经序列测定和软件分析比较,发现这些片段所编码的蛋白质氨基酸序列,都有CHITINASE-19.1和CHITINASE-19.2的基序,为第II类几丁质酶基因。扩增的核酸序列在GenBank上发表,登录号分别为AY973229和AY973230。
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| 关 键 词： | 小麦种子 几丁酶基因 扩增 序列分析 |
| 文章编号： | 1672-5565（2007）-01-01-04 |
| 修稿时间： | 2006-02-14 |
Gene cloning and sequence analysis of chitinase gene in wheat seed |
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| YI Tu-yong,Tatsuya Ikeda. Gene cloning and sequence analysis of chitinase gene in wheat seed[J]. Chinese Journal of Bioinformatics, 2007, 5(1): 1-4 |
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| Authors: | YI Tu-yong Tatsuya Ikeda |
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| Affiliation: | 1.College of Bio-safety Science and Technology; Hunan Agricultural University; Changsha 410128; China; 2.National Agricultural Research Center for West Region; Fukuyama 062-8555; Japan |
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| Abstract: | Based on chitinase gene-relative TC187877 from http://www.tigr.org,two pairs of primers were designed,and two 1000bp fragments were amplified from seed genomic DNA of Gamenya and Sumai 3 by PCR method.The two amplified fragments belong to Chitinase II because of their CHITINASE-19.1and CHITINASE-19.2 motifs at the amino acid level through sequencing and sequence analysis.The amplified sequences were deposited in GenBank(GenBank accession number AY973229 and AY973230 respectively). |
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| Keywords: | Wheat seed chitinase gene amplify sequence analysis |
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