Purification and Characterization of Glycerol Dehydratase from Lactobacillus reuteri |
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Authors: | Todd L. Talarico and Walter J. Dobrogosz |
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Abstract: | A coenzyme B12-dependent glycerol dehydratase from Lactobacillus reuteri has been purified and characterized. The dehydratase has a molecular weight of approximately 200,000, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis yielded a single major band with a molecular weight of 52,000. Km values for substrates and coenzyme B12 were in the millimolar and the submicromolar range, respectively. |
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