不同烤烟基因型钾离子通道特性研究

通过对4个不同钾营养效率的烤烟品种的根皮层细胞质膜内向跨膜钾电流进行全细胞记录,研究钾营养效率不同的烤烟基因型的钾离子通道的特性。结果表明,NC89的电流密度(pA/pF)最高,约为-80;其次是农大202及净叶黄,其电流密度分别为-60及-50;NC2326的电流密度在四个基因型中最小,大约-40。结果表明,烤烟根皮层细胞质膜跨膜内向钾电流的大小,与基因型对K+亲和力及吸收能力的大小,以及烤烟基因型钾营养效率高低有着密切的关系,可以作为一个比较可靠的参考鉴定指标,指导K+营养高效的基因型的筛选与鉴定。

Characteristics of potassium channel of different tobacco genotypes

The characteristics of root cortex protoplast whole cell transmembrane in-ward K+ current of four flue-cured tobacco genotypes with different potassium use efficiency were studied. The results showed that the current density (pA/pF) of NC89 (-80) was the highest of all four genotypes,then Nongda 202,Jingyehuang and NC2326 were -60,-50 and -40 respectively. The results suggested that the current density (pA/pF) of transmembrane in-ward K+ current was closely related to the potassium absorption ability and potassium use efficiency of flue-cured tobacco,and could be used as a effective method to screen high potassium use efficiency flue-cured tobacco genotype.