Characterization of 47 MHC class I sequences in Filipino cynomolgus macaques |
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Authors: | Kevin J Campbell Ann M Detmer Julie A Karl Roger W Wiseman Alex J Blasky Austin L Hughes Benjamin N Bimber Shelby L O’Connor David H O’Connor |
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Institution: | (1) Wisconsin National Primate Research Center, University of Wisconsin—Madison, Madison, WI 53706, USA;(2) Department of Biological Sciences, University of South Carolina, Columbus, SC 29208, USA;(3) Department of Pathology and Laboratory Medicine, University of Wisconsin—Madison, Madison, WI 53706, USA;(4) University of Wisconsin—Madison, 555 Science Drive, Madison, WI 53711, USA |
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Abstract: | Cynomolgus macaques (Macaca fascicularis) provide increasingly common models for infectious disease research. Several geographically distinct populations of these
macaques from Southeast Asia and the Indian Ocean island of Mauritius are available for pathogenesis studies. Though host
genetics may profoundly impact results of such studies, similarities and differences between populations are often overlooked.
In this study we identified 47 full-length MHC class I nucleotide sequences in 16 cynomolgus macaques of Filipino origin.
The majority of MHC class I sequences characterized (39 of 47) were unique to this regional population. However, we discovered
eight sequences with perfect identity and six sequences with close similarity to previously defined MHC class I sequences
from other macaque populations. We identified two ancestral MHC haplotypes that appear to be shared between Filipino and Mauritian
cynomolgus macaques, notably a Mafa-B haplotype that has previously been shown to protect Mauritian cynomolgus macaques against challenge with a simian/human immunodeficiency
virus, SHIV89.6P. We also identified a Filipino cynomolgus macaque MHC class I sequence for which the predicted protein sequence differs from
Mamu-B*17 by a single amino acid. This is important because Mamu-B*17 is strongly associated with protection against simian immunodeficiency virus (SIV) challenge in Indian rhesus macaques. These
findings have implications for the evolutionary history of Filipino cynomolgus macaques as well as for the use of this model
in SIV/SHIV research protocols.
Kevin J. Campbell and Ann M. Detmer contributed equally to this work. |
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Keywords: | MHC Filipino Macaca fascicularis SIV |
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