Determination of ketone body turnover in vivo with stable isotopes, utilizing gas chromatography/mass spectrometry |
| |
Authors: | J M Miles W F Schwenk K L McClean M W Haymond |
| |
Institution: | Department of Biochemistry, The University of Iowa, Iowa City, Iowa 52242 USA |
| |
Abstract: | JH imple and reliable method for the determination of ketone body turnover in vivo using a primed, continuous infusion of 3,4-13C2]acetoacetate is described. Mole percent enrichment of beta-13C2]hydroxybutyrate and 13C2]acetoacetate is determined by gas chromatography/mass spectrometry using electron-impact ionization and selected ion monitoring. Ketone body flux data are provided from preliminary dog experiments. The method is readily applicable to the study of ketone body metabolism in both laboratory animals and humans. |
| |
Keywords: | To whom correspondence should be addressed |
本文献已被 ScienceDirect PubMed 等数据库收录! |