Comparison of chloroplast DNAs by specific fragmentation with EcoRI endonuclease |
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| Authors: | Bentley A Atchison Paul R Whitfeld and Warwick Bottomley |
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| Institution: | (1) Department of Molecular Cell Biology Section Microbiology, State University, Transitorium 3, Padualaan 8, Utrecht, The Netherlands;(2) Section Electron Microscopy, State University, Transitorium 3, Padualaan 8, Utrecht, The Netherlands;(3) Institute for Molecular Biology, State University, Transitorium 3, Padualaan 8, Utrecht, The Netherlands |
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| Abstract: | Summary Mutants ofEscherichia coli K12, deficient in up to three major outer membrane proteinsb,c andd have been constructed. Mutants that lack the lipopolysaccharide sugar heptose are deficient in proteinb. All heptose-deficient strains are supersensitive to lysozyme, various antibiotics and detergents. They excrete the periplasmic enzyme ribonuclease I. Mutants deficient in proteinsc and/ord have the same sensitivity towards these compounds as the parent strain. Cells of single, double and triple mutants are all rod-shaped. Electrophoretic analysis of cell evelope proteins indicates that in some mutants the protein deficiency is partially compensated for by increased amounts of one or two of the other major outer membrane proteins. Heptose-deficient strains have an increased amount of 2-keto-3-deoxyoctonate. |
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