Effect of in vitro selenium supplementation on bovine sperm motility |
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Authors: | Siegel R B Murray F A Julien W E Moxon A L Conrad H R |
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Affiliation: | Department of Animal Science Ohio Agricultural Research and Development Center Wooster, Ohio 44691 USA; Department of Dairy Science Ohio Agricultural Research and Development Center Wooster, Ohio 44691 USA. |
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Abstract: | The effects of selenium (Se) on motility of bovine spermatozoa were evaluated in two experiments. In Experiment 1, increasing doses of Se alone or with vitamin E were found to increase motility in Se concentrations up to 1 mug/ml. However, Se alone had a greater effect than did Se with vitamin E. The sperm motility response to Se concentrations from background to 1 mug/ml on motility best fit a quadratic equation with a maximum at about .65 mug Se/ml. In Experiment 2, the effect of Se addition before and after freezing was evaluated. This experiment demonstrated that treatment of semen with Se before freezing significantly increased the post-thaw motility as compared with portions of the same ejaculate which were not treated. Treatment after thawing produced motilities which were intermediate and not significantly different from either pre-freezing supplementation or no supplementation at all. These data indicate that some semen exhibits a greater percentage of motile sperm when Se is added prior to freezing, however, no information is yet available on the fertility of Se-treated semen. |
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