Field detection of Schistosoma japonicum cercariae in environmental water samples by quantitative PCR |
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| Authors: | Worrell Caitlin Xiao Ning Vidal Jorge E Chen Lin Zhong Bo Remais Justin |
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| Affiliation: | Department of Environmental Health, Rollins School of Public Health, Emory University, 1518 Clifton Rd. NE, Atlanta, Georgia 30322,1 Institute of Parasitic Disease, Sichuan Center for Disease Control and Prevention, Chengdu, Sichuan 61004, People''s Republic of China,2 Hubert Department of Global Health, Rollins School of Public Health, Emory University, 1518 Clifton Rd. NE, Atlanta, Georgia 303223 |
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| Abstract: | A species-specific quantitative PCR (qPCR) assay was combined with two novel water-sampling methods and compared with the mouse bioassay for the quantitative detection of S. japonicum in surface waters. The novel methods were capable of capturing cercariae and, with subsequent analysis through qPCR, detecting the presence of a minimum of 1 cercaria. |
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