Good agreements between self and clinician-collected specimens for the
detection of human papillomavirus in Brazilian patients |
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Authors: | Karla Lopes Mandu de Campos Ana Paula Machado Flávia Gatto de Almeida Camila Mareti Bonin Thiago Theodoro Martins Prata Larissa Zatorre Almeida Cacilda Tezelli Junqueira Padovani Alda Maria Teixeira Ferreira Carlos Eurico dos Santos Fernandes Inês Aparecida Tozetti |
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Institution: | 1. Laboratório de Imunologia e Biologia Molecular de Vírus, Centro de Ciências Biológicas e da Saúde;2. Programa de Pós-Graduação em Doenças Infecciosas e Parasitárias, Faculdade de Medicina, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brasil |
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Abstract: | Women infected with human papillomavirus (HPV) are at a higher risk of developing
cervical lesions. In the current study, self and clinician-collected vaginal and
cervical samples from women were processed to detect HPV DNA using polymerase chain
reaction (PCR) with PGMY09/11 primers. HPV genotypes were determined using
type-specific PCR. HPV DNA detection showed good concordance between self and
clinician-collected samples (84.6%; kappa = 0.72). HPV infection was found in 30%
women and genotyping was more concordant among high-risk HPV (HR-HPV) than low-risk
HPV (HR-HPV). HPV16 was the most frequently detected among the HR-HPV types. LR-HPV
was detected at a higher frequency in self-collected; however, HR-HPV types were more
frequently identified in clinician-collected samples than in self-collected samples.
HPV infections of multiple types were detected in 20.5% of clinician-collected
samples and 15.5% of self-collected samples. In this study, we demonstrated that the
HPV DNA detection rate in self-collected samples has good agreement with that of
clinician-collected samples. Self-collected sampling, as a primary prevention
strategy in countries with few resources, could be effective for identifying cases of
HR-HPV, being more acceptable. The use of this method would enhance the coverage of
screening programs for cervical cancer. |
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Keywords: | human papillomavirus self-collected clinician-collected PCR |
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