| Abstract: | Giardia duodenalis is one of the most prevalent enteroparasites in
children. This parasite produces several clinical manifestations. The aim of this
study was to determine the prevalence of genotypes of G. duodenalis
causing infection in a region of southeastern Mexico. G. duodenalis
cysts were isolated (33/429) from stool samples of children and molecular genotyping
was performed by polymerase chain reaction-restriction fragment length polymorphism
(PCR-RFLP) analysis, targeting the triosephosphate isomerase ( tpi )
and glutamate dehydrogenase ( gdh ) genes. The tpi
gene was amplified in all of the cyst samples, either for assemblage A (27 samples)
or assemblage B (6 samples). RFLP analysis classified the 27 tpi -A
amplicons in assemblage A, subgenotype I. Samples classified as assemblage B were
further analysed using PCR-RFLP of the gdh gene and identified as
assemblage B, subgenotype III. To our knowledge, this is the first report of
assemblage B of G. duodenalis in human clinical samples from
Mexico. |
