Studies on the length of the side chains of the dextran elaborated by Leuconostoc mesenteroides NRRL B-512 |
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| Authors: | O Larm B Lindberg S Svensson |
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| Affiliation: | 1. Department of Mechanical Engineering, Chung Yuan Christian University, Chung Li 32023, Taiwan;2. Center for Biomedical Technology (CBT), Chung Yuan Christian University, Chung Li 32023, Taiwan;1. Department of Biological Physical Chemistry, Institute of Physical Chemistry “Rocasolano”, CSIC, Serrano 119, 28006, Madrid, Spain;2. Department of Biochemistry and Molecular Biology I, Faculty of Chemistry, Complutense University, 28040, Madrid, Spain;1. Universität Leipzig, Johannisallee 29, 04103 Leipzig, Germany;2. FR 8.1 Universität des Saarlandes, Postach 151150, 66041 Saarbrücken, Germany;3. School of Materials Science and Engineering, Tianjin University, Tianjin 300072, People’s Republic of China;4. Helmholtz-Zentrum Berlin für Materialien und Energie, Hahn-Meitner-Platz 1, 14109 Berlin, Germany;1. CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Chaoyang District, Beijing 100101, China;2. Lanzhou Institute of Animal Science and Veterinary Pharmaceutics, Chinese Academy of Agricultural Science, Lanzhou 730050, China;3. Laboratory of Tumor Biology and Glycobiology, Shenyang Pharmaceutical University, Shenyang 110016, China;4. Savaid Medical School, University of Chinese Academy of Sciences, Huairou District, Beijing 101408, China;5. Center for Influenza Research and Early-warning, Chinese Academy of Sciences (CASCIRE), Chaoyang District, Beijing 100101, China;3. College of Agriculture, Food and Environment Sciences, Rakuno Gakuen University, Ebetsu 069-8501 and;4. Research Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan;1. Department of Biobased Materials Science, Graduate School of Science and Technology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan;2. Department of Chemistry, Biotechnology and Chemical Engineering, Graduate School of Science and Engineering, Kagoshima University, 1-21-40 Korimoto, Kagoshima 860-0065, Japan |
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| Abstract: | The length of the side chains in dextran NRRL B-512 has been studied by sequential degradation. Selective removal of the terminal d-glucosyl groups was achieved by alkaline treatment of a methylated dextran in which the hydroxymethyl groups had been replaced by C-p-tolylsulfonylmethyl groups; the 6-hydroxyl group of the new terminal units thus formed is unsubstituted. The degraded material was subjected to a second degradation by using the same procedure. The reactions were followed by methylation and/or ethylation of the products, acid hydrolysis, and analysis of the resulting mixture of ethers by g.l.c.-m.s. As a result of these studies, it is concluded that about 40% of the side chains contain only one d-glucose residue, at least 45% are two d-glucose units long, and the rest (max. 15%) are longer than two such units. |
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