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New Trichobrachins, 11-residue peptaibols from a marine strain of Trichoderma longibrachiatum
Authors:Ruiz Nicolas  Wielgosz-Collin Gaëtane  Poirier Laurence  Grovel Olivier  Petit Karina Ethel  Mohamed-Benkada Mustapha  du Pont Thibaut Robiou  Bissett John  Vérité Philippe  Barnathan Gilles  Pouchus Yves François
Institution:Université de Nantes, Nantes Atlantique Universités, SMAB-EA 2160, Faculté de pharmacie, BP 53508, F-44000 Nantes, France.
Abstract:A marine strain of Trichoderma longibrachiatum isolated from blue mussels (Mytilus edulis) was investigated for short peptaibol production. Various 11-residue peptaibols, obtained as microheterogenous mixtures after a chromatographic fractionation, were identified by positive mass spectrometry fragmentation (ESI-IT-MS(n), CID-MS(n) and GC/EI-MS). Thirty sequences were identified, which is the largest number of analogous sequences so far observed at once. Twenty-one sequences were new, and nine others corresponded to peptaibols already described. These peptaibols belonged to the same peptidic family based on the model Ac-Aib-xxx-xxx-xxx-Aib-Pro-xxx-xxx-Aib-Pro-xxol. They were named trichobrachin A when the residue in position 2 was an Asn, and trichobrachin C when it was a Gln. Major chromatographic sub-fractions, corresponding to purified peptaibols, were assayed for their cytotoxic activity. Trichobrachin A-IX and trichobrachin C exhibited the highest activities. There was an exponential relation between their relative hydrophobicity and their cytotoxicity on KB cells.
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