| 应用DNA-RNA斑点杂交法检测登革病毒核酸 |
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| 引用本文： | 陈火胜,郭辉玉,罗进贤,胡晋新. 应用DNA-RNA斑点杂交法检测登革病毒核酸[J]. Virologica Sinica, 1989, 0(4) |
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| 作者姓名： | 陈火胜 郭辉玉 罗进贤 胡晋新 |
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| 作者单位： | 中山医科大学微生物学与免疫学教研室 广州(陈火胜，郭辉玉)，中山大学生物工程研究中心 广州(罗进贤，胡晋新) |
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| 摘 要： | 新近制备了大量纯化的pEH920 DNA,该质粒DNA插入了登革病毒2型核酸片段的互补DNA。以[a-~(32)P]dCTP按缺口转译法标记pEH 920 DNA作为探针,以感染病毒的蚊细胞c_6/36培养上清作标本,应用DNA-RNA斑点杂交法检测了登革病毒核酸。结果显示同位素标记探针(pEH 920)与登革病毒2型标本反应最强,具有一定的型特异性。但与其它血清型登革病毒也呈一定交叉反应。初步探讨了探针的敏感性,至少可检出TCID_(50)625的登革病毒2型核酸。
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| 关 键 词： | 登革病毒 缺口转译 斑点杂交 |
Detection of Dengue Virus RNA by DNA-RNA Dot-spot Hybridization |
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| Chen Huo-sheng Guo Hui-yu. Detection of Dengue Virus RNA by DNA-RNA Dot-spot Hybridization[J]. 中国病毒学(英文版), 1989, 0(4) |
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| Authors: | Chen Huo-sheng Guo Hui-yu |
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| Abstract: | Recently we have prepared large amount of purified pEH 920 DNA which is inserted by complementary DNA of dengue virus 2 RNA. Using the [a-~(32)p] dCTP labelled pEH 920 DNA as the probe, dengue virus RNA in the supernatants of infected C6/36 mosquito cells have been detected by DNA-RNA dot-spot hybridization. The results show that the probe strongly reacted with dengue 2 virus RNA though it also cross-hybridized with dengue 1, 3 and 4 virus RNA. The preliminary experiments show that the probe can at least detect 625 TCID_(50) of dengue 2 virus RNA. The Chinese isolates of dengue 2 virus can also be detected by the probe. |
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| Keywords: | Dengue virus Nick translation Dot-spot hybridization |
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