Effects of pH and corn steep liquor variability on mannitol production by Lactobacillus intermedius NRRL B-3693 |
| |
Authors: | Badal C Saha F Michael Racine |
| |
Institution: | (1) Bioenergy Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, US Department of Agriculture, Peoria, IL 61604, USA;(2) zuChem, Inc., Peoria, IL 61604, USA |
| |
Abstract: | Lactobacillus intermedius NRRL B-3693 produced mannitol, lactic acid, and acetic acid when grown on fructose at 37°C. The optimal pH for mannitol production
from fructose by the heterofermentative lactic acid bacterium (LAB) in pH-controlled fermentation was at pH 5.0. It produced
160.7 ± 1.1 g mannitol in 40 h with a volumetric productivity of 4.0 g l−1 h−1 in a simplified medium containing 250 g fructose, 50 g corn steep liquor (CSL), and 33 mg MnSO4 per liter. However, the mannitol production by the LAB was severely affected by the variability of CSL. The supplementation
of CSL with soy peptone (5 g/l), tryptophan (50 mg/l), tryptophan (50 mg/l) plus tyrosine (50 mg/l), or commercial protease
preparation (2 ml/100 g of CSL) enhanced the performance of the inferior CSL and thus helped to overcome the nutrient limitations. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|