Characterization of Bombyx mori Nucleopolyhedrovirus ORF109 that Encodes a 25-kDa Structural Protein of the Occlusion-Derived Virion |
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| Authors: | Yang Zhou Qin Yao Hongxing Shen Hengchuan Xia Feng Lin Keping Chen |
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| Affiliation: | (1) Institute of Life Sciences, Jiangsu University, 301# Xuefu Road, Zhenjiang, 212013, People’s Republic of China;(2) School of Medical Science and Laboratory Medicine, Jiangsu University, 301# Xuefu Road, Zhenjiang, 212013, People’s Republic of China |
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| Abstract: | Bombyx mori nucleopolyhedrovirus (BmNPV) ORF109 (Bm109) is a gene present in many lepidopteran NPVs, but its function is unknown. In this study, Bm109 was characterized. The transcript of Bm109 was detected at 12–72 h postinfection (p.i.). Polyclonal antiserum raised to a His-BM109 fusion protein recognized BM109 in infected cell lysates from 24 to 72 h p.i., suggesting that Bm109 is a late gene. Localization of the BM109 in BV and ODV by western analyses demonstrated that BM109 was proteins of ODV. |
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