Mutants of Bacillus subtilis Bacteriophage φe Defective in dTTP-dUTP Nucleotidohydrolase |
| |
| Authors: | Linda T Dunham and Alan R Price |
| |
| Institution: | aDepartment of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan 48104 |
| |
| Abstract: | Mutants of bacteriophage phie inducing only 1 to 5% of wild-type levels of dTTP-dUTP nucleotidohydrolase give normal bursts of viable progeny phage whose DNA contains 5 to 10% thymine (but no uracil) in place of 5-hydroxymethyluracil. The relative heat lability of one phage mutant enzyme solubilized from the membrane fraction of infected cells suggests that a phie gene codes for the induced dTTPase-dUTPase. |
| |
| Keywords: | |
|
