双氯醇胺对湖羊后肢肌肉氨基酸净平衡的调节

采用４头装有长久性肠系膜静脉和右后肢股动、静脉血管瘘的湖羊，按自身对照设计，研究了β－激动剂双氯醇胺以湖羊后肢肌肉氨基酸净平衡的调节。经肠系膜静脉灌注双氯醇胺，湖羊后肢血液氨基酸流量与对照其相比有有显著差异（Ｐ〈０．０１）。在１５种氨基酸中，有１０种氨基酸的吸收量大幅度增加；另有５种氨基酸释放量增加，或者吸收量减小。结果表明，有氯醇胺可通过改变肌肉吸收或释放氨基酸的种类和数量来调节湖羊后肢肌肉氨基

CLENBUTEROL'S REGULATION EFFECTS ON AMINO ACID NET BALANCE IN HU SHEEP'S HINDQUARTER MUSCLE

Four Hu sheep fitted with chronic catheters at mesenteric vein and right hindquarter artery and vein were used to study the effect of clenbuterol on the amino acid net balance by means of determining the amino acid flow through their right hindquarter muscle. The results are as follows. In the control period, the total amino acid flows of the right hindquarter vein and artery were 4 54 mg/min and 5 48 mg/min, respectively. Compared with the control period, after injecting clenbuterol into the mesenteric vein, amino acid flow in right hindquarter vein and artery increased greatly( P <0 01): 7 70 mg/min in the vein, 9 61 mg/min in the artery, respectively. Of the 15 amino acids determined,in the experiment group,besides an obvious increase of the flow of all amino acids, a distinct increase could be found in the total quantity for 10 amino acids absorbed (Asp,Glu,Thr,Gly,Ala,Pro,Met,Val,Leu and Lys)and 3 amino acids released(Arg, His and Ser)by the hindquarter muscle( P <0 05). This indicated that clenbuterol could regulate amino acid net balance by altering the absorption or release of amino acids.