Comparing protocols for preparation of DNA-free total yeast RNA suitable for RT-PCR期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Comparing protocols for preparation of DNA-free total yeast RNA suitable for RT-PCR

Authors:	Eduardo?M?Del Aguila,Marcio?B?Dutra,Joab?T?Silva,Vania?MF?Paschoalin author-information" > author-information__contact u-icon-before" > mailto:paschv@iq.ufrj.br" title=" paschv@iq.ufrj.br" itemprop=" email" data-track=" click" data-track-action=" Email author" data-track-label=" " >Email author

Affiliation:	1.Departamento de Bioquímica, Instituto de Química,Universidade Federal do Rio de Janeiro, Centro de Tecnologia,Rio de Janeiro,Brasil;2.Centro de Ciências da Saúde, Universidade Salgado de Oliveira,S?o Gon?alo,Brasil

Abstract:	Background Preparation of RNA free from DNA is a critical step before performing RT-PCR assay. Total RNA isolated from several sources, including those obtained from Saccharomyces cerevisiae, using routine methodologies are frequently contaminated with DNA, which can give rise to amplification products that mimic the amplicons expected from the RNA target.

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