Detection of a plant virus in cryosections of its host plant |
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| Affiliation: | 1. Instituto di Fitovirologia Applicata del CNR, Via O. Vigliani 104, 10135 Torino, Italy;2. Universität des Saarlandes, 665 Homburg, Fachr. 3.5, F.R.G.;1. Australian Centre for Microscopy and Microanalysis, University of Sydney, Madsen Building F09, NSW 2006, Australia;2. Department of Materials, University of Oxford, Parks Road, Oxford, OX1 3PH, United Kingdom;3. Physical and Computational Sciences Directorate, Pacific Northwest National Laboratory, P.O. Box 999, Richland, WA 99352, USA;4. Environmental Molecular Science Laboratory, Pacific Northwest National Laboratory, P.O. Box 999 Richland, WA 99352, USA;5. School of Aerospace, Mechanical and Mechatronic Engineering, University of Sydney, NSW 2006, Australia;1. ZJU-Hangzhou Global Scientific and Technological Innovation Centre, Zhejiang University, Hangzhou 311200, PR China;2. State Key Laboratory of Resources and Environment Information System, Institute of Geographical Sciences & Natural Resources Research, Chinese Academy of Sciences, Beijing 100871, PR China;3. School of Materials Science and Engineering, Peking University, Beijing 100871, PR China;4. Centre for Clean Energy Technology, School of Mathematical and Physical Sciences, University of Technology Sydney, Sydney, NSW 2007 Australia;5. School of Engineering, RMIT University, 124 La Trobe Street, Melbourne, Victoria 3001, Australia;6. Department of Materials Engineering, KU Leuven Kasteelpark Arenberg 44, Bus 2450, B-3001 Heverlee, Belgium;7. International Center for Quantum Materials and Electron Microscopy Laboratory, School of Physics, Peking University, Beijing 100871, PR China;8. College of Chemical and Biological Engineering, State Key Laboratory of Chemical Engineering, Zhejiang University, Hangzhou 310027, PR China;9. Institute of Science and Technology for New Energy, Xi''an Technological University, Xi''an 710021, PR China;10. School of Materials Science and Engineering, Zhejiang University, Hangzhou 310027, PR China;11. State Key Laboratory of Baiyunobo Rare Earth Resource Researches and Comprehensive Utilization, Baotou Research Institute of Rare Earths, Baotou, 014030 PR China;1. Department of Pharmacognosy, Faculty of Pharmacy, Ataturk University, Erzurum, Turkey;2. Department of Chemistry, Faculty of Science, Ataturk University, Erzurum, Turkey;3. Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Ataturk University, Erzurum, Turkey;4. Department of Biology, Faculty of Science, Ankara University, Ankara, Turkey;5. Department of Pharmacognosy, Faculty of Pharmacy, Anadolu University, Eskisehir, Turkey;6. Department of Plant Physiology, Slovak University of Agriculture, Nitra, Slovak Republic;7. Department of Botany and Plant Physiology, Faculty of Agrobiology, Food and Natural Resources Czech University of Life Sciences, 16500 Prague, Czech Republic;8. Department of Plant Biology, Institute of Biology, Kiev National University of Taras Shevchenko, Volodymyrska, 64, Kyiv, Ukraine;1. HainanProvincial Key Laboratory of Resources Conservation and Development of Southern Medicine, Hainan Branch Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Wanning 571533, China;2. National Engineering Laboratory for Breeding of Endangered Medicinal Materials, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China |
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| Abstract: | The technique of cryo-ultramicrotomy was used for the detection of a plant reovirus in the tissue of its host plant. The observations made on cryosections, compared to those made on conventional sections, show that virus and related structures are well preserved, and that this technique can be usefully employed in plant virology. |
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