Cytokeratin filament expression during in vitro teratocarcinoma cell differentiation as detected by a monoclonal antibody |
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Affiliation: | 1. Dept of Pathology, St. Radboud Hospital, University of Nijmegen The Netherlands;2. Dept of Cell Biology and Genetics, Erasmus University of Rotterdam, The Netherlands |
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Abstract: | Undifferentiated F9 teratocarcinoma cells were induced to differentiate in culture using retinoic acid and cAMP. As a result the morphology of the cultures changes dramatically. Using a monoclonal antibody directed against cytokeratin polypeptide 18 (RGF 53) in the indirect immunofluorescence technique we could show that this cytokeratin subunit is synthesized and assembled into a filamentous network upon differentiation in about 50% of the cells. Immunoblotting studies confirm these results. |
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