Tetrahedral framework nucleic acids promote diabetic wound healing via the Wnt signalling pathway |
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| Authors: | Zejing Wang Hao Lu Tao Tang Lei Liu Bohan Pan Jiqiu Chen Dasheng Cheng Xiaoxiao Cai Yu Sun Feng Zhu Shihui Zhu |
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| Affiliation: | 1. Burn Institute of PLA, Department of Burn Surgery, the First Affiliated Hospital of Naval Medical University, Research Unit of Key Techniques for Treatment of Burns and Combined Burns and Trauma Injury, Chinese Academy of Medical Sciences, Shanghai China ; 2. State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu China |
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| Abstract: | ObjectivesTo determine the therapeutic effect of tetrahedral framework nucleic acids (tFNAs) on diabetic wound healing and the underlying mechanism.Materials and MethodsThe tFNAs were characterized by polyacrylamide gel electrophoresis (PAGE), atomic force microscopy (AFM), transmission electron microscopy (TEM), dynamic light scattering (DLS) and zeta potential assays. Cell Counting Kit‐8 (CCK‐8) and migration assays were performed to evaluate the effects of tFNAs on cellular proliferation and migration. Quantitative polymerase chain reaction (Q‐PCR) and enzyme‐linked immunosorbent assay (ELISA) were used to detect the effect of tFNAs on growth factors. The function and role of tFNAs in diabetic wound healing were investigated using diabetic wound models, histological analyses and western blotting.ResultsCellular proliferation and migration were enhanced after treatment with tFNAs in a high‐glucose environment. The expression of growth factors was also facilitated by tFNAs in vitro. During in vivo experiments, tFNAs accelerated the healing process in diabetic wounds and promoted the regeneration of the epidermis, capillaries and collagen. Moreover, tFNAs increased the secretion of growth factors and activated the Wnt pathway in diabetic wounds.ConclusionsThis study indicates that tFNAs can accelerate diabetic wound healing and have potential for the treatment of diabetic wounds.Tetrahedral framework nucleic acids (tFNAs) can facilitate the proliferation and migration of HaCaTs, HDFs and HUVECs in a high‐glucose environment. tFNAs can accelerate diabetic wound healing by promoting epithelialization, vascularization, collagen synthesis and the secretion of growth factors via the Wnt pathway. |
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