Enzymes of nucleic acid metabolism as biochemical markers of T cell development in mouse |
| |
| Affiliation: | 1. Department of Chemistry, Princeton University, Princeton, NJ, USA;2. Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ, USA;3. Ludwig Institute for Cancer Research, Princeton Branch, Princeton University, Princeton, NJ, USA;1. Helmholtz Center Munich; German Research Center for Environmental Health, Munich, Germany;2. Department of Medicine III and Transfusion Medicine, University Hospital Grosshadern, Ludwig-Maximilians-University, Munich, Germany |
| |
| Abstract: | The levels of three purine salvage enzymes, adenosine deaminase (ADA), purine nucleoside phosphorylase (PNP) and 5′-nucleotidase (5′-N), which are known to be associated with certain immunodeficiency disorders were determined in mouse T lymphocytes. These enzymes showed characteristic changes depending on the stage of T cell development. The activity of ADA was 5-fold higher in thymocytes compared to splenic T cells. On the other hand, the splenic T cells displayed a 2-fold and a 4–8 fold greater activity of PNP and 5′-N, respectively than those of thymocytes. The apparent Km and Vmax values have been determined for all the 3 enzymes in the immature and mature T cells. The data demonstrate that the absolute and relative activity of these enzymes may be used as biochemical markers to characterize the T lymphocytes during different stages of differentiation. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
