Development and validation of an LC-MS/MS method for determination of 8-iso-prostaglandin f2 Alpha in human saliva |
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Authors: | Zlatina Tomova Desislav Tomov Angelina Vlahova Veneta Chaova-Gizdakova Lyubka Yoanidu Dobrin Svinarov |
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Affiliation: | 1. Medical University of Plovdiv, Faculty of Dental Medicine, Department of Prosthetic Dental Medicine, Plovdiv, Bulgaria ; 2. Medical University of Plovdiv, Faculty of Pharmacy, Department of Bioorganic Chemistry, Plovdiv, Bulgaria ; 3. Research Institute at Medical University of Plovdiv, Plovdiv, Bulgaria ; 4. Medical University of Sofia, Faculty of Medicine, Clinical Laboratory & Clinical Pharmacology, UMBAL Alexandrovska, Sofia, Bulgaria |
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Abstract: | BackgroundIncreased formation of reactive oxygen species may be caused by the ion release of the metal alloys used in prosthetic dental restorations due to the corrosion process. As products of lipid peroxidation, isoprostanes can be used as a marker for oxidative stress in the body. There are two significant advantages of using isoprostanes as an oxidative stress marker - presence in all fluids in the body and low reactivity. Saliva provides noninvasive, painless, and cost-effective sample collection and can be used as an alternative testing medium of blood and urine.MethodsThis study presents the development and validation of a sample LC-MS/MS method to quantify 8-isoprostaglandin F2-a in human saliva using salt-out assisted liquid-liquid extraction (SALLE).ResultsThe selected sample preparation procedure optimized chromatographic separation and mass detection provided high recovery and sensitivity of the analysis. The calibration curve was obtained in the predefined range 25-329 ng/L with R2 larger than 0.995. Normalized matrix varied between 89.7 % and 113.5%. The method showed sufficient accuracy and precision - accuracy in the range 89.7 %-113.9 %, and precision between 2.3% and 5.4%.ConclusionsThe proposed method is validated according to current EMA/FDA industrial guidance for bioanalysis and offers an appropriate level of sensitivity and sufficient accuracy and precision. |
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Keywords: | metal alloy 8-isoPGF2a LC-MS/MS saliva SALLE |
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