High-performance liquid chromatographic analysis of isoxicam in human plasma and urine |
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| Affiliation: | 1. Mahidol Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand;2. Centre for Tropical Medicine and Global Health, Nuffield Department of Clinical Medicine, University of Oxford, Oxford, UK;1. Applied Environmental Research Laboratories, Department of Chemistry, Vancouver Island University, Nanaimo, BC, Canada;2. Department of Chemistry, University of Victoria, Victoria, BC, Canada;3. Fraser Health Authority, Vancouver, BC, Canada;4. Department of Chemistry, Simon Fraser University, Burnaby, BC, Canada;5. Department of Environmental and Occupational Health Sciences, University of Washington, Seattle, WA, USA;6. Canadian Institute for Substance Use Research (CISUR), University of Victoria, Victoria, BC, Canada |
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| Abstract: | A sensitive, selective, and rapid high-performance liquid chromatographic procedure was developed for the determination of isoxicam in human plasma and urine. Acidified plasma or urine were extracted with toluene. Portions of the organic extract were evaporated to dryness, the residue dissolved in tetrahydrofuran (plasma) or acetonitrile (urine) and chromatographed on a μBondapak C18 column preceded by a 4–5 cm × 2 mm I.D. column packed with Corasil C18. Quantitation was obtained by UV spectrometry at 320 nm. Linearity in plasma ranged from 0.2 to 10 μg/ml. Recoveries from plasma samples seeded with 1.8, 4 and 8 μg/ml isoxicam were 1.86 ± 0.077, 4.10 ± 0.107 and 8.43 ± 0.154 μg/ml with relative standard deviations of 3.3%, 2.5% and 5.4%, respectively. The linearity in urine ranged from 0.125 to 2 μg/ml. The precision of the method was 3.3–9.0% relative standard deviation over the linear range. |
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